I= PART-1: CHARACTERIZATION OF HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICKII PART-II: PROTEIN PATTERNS OF HUMAN CATARACTOUS LENS AND PLASMA
Pakistan Research Repository Home
 

Title of Thesis
PART-1: CHARACTERIZATION OF HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICKII PART-II: PROTEIN PATTERNS OF HUMAN CATARACTOUS LENS AND PLASMA

Author(s)
Rukhsana Lalani
Institute/University/Department Details
University f Karachi/ H.E.J. Research Institute of Chemistry
Session
1984
Subject
Chemistry
Number of Pages
117
Keywords (Extracted from title, table of contents and abstract of thesis)
hemoglobin, reptile uromastix hardwickii, protein patterns, human cataractous lens, plasma, hemoglobin concentration, senile cataract

Abstract
The present study on hemoglobin of Uromastix hardwickii has been undertaken to separate and characterize hemoglobin components. The separation of hemoglobin components was achieved by subjecting it to Sephadex G-75 column chromatography, ion-exchange chromatography using different resins and variety of buffers. The separation on molecular weight basis gave four peaks but when their homogeneity was checked, it was found that all the four peaks were not pure components. The separation on charge basis resolved into number of peaks. Checking the purity of these peaks by triton electrophoresis and N-terminal analysis showed only two of them to be pure. The purity of the same were checked on HPLC showing them to be homogeneous. However, the amino acid sequence using DABITC method could be achieved only to a limited number

Download Full Thesis
727.5 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents 0
44.14 KB
2 1 Part-I: Characterization Of Hemoglobin From The Reptile Uromastix Hardwickii 1
129.87 KB
  1.1 Introduction Hemoglobin 1
  1.2 Function Of Hemoglobin 1
  1.3 Structure Of Hemoglobin 4
  1.4 Primary Structure Of Vertebrate Hemoglobin 6
  1.5 Reptile Hemoglobin 7
  1.6 Evolution And Phylogeny 11
  1.7 Objectives Of The Study 17
3 2 Materials And Methods 18
154.75 KB
  2.1 Blood From Uromastix 18
  2.2 Extraction Of Hemoglobin 18
  2.3 Photometric Estimation Of Hemoglobin Concentration 18
  2.4 Separation Of Heme 19
  2.5 Gel Filtration 19
  2.6 Ion-Exchange Charomatography 21
  2.7 Purity On Hplc 23
  2.8 Electrophoresis 23
  2.9 Oxidation With Performic Acid 29
  2.10 Amino Acid Analysis 29
  2.11 N-Terminal Sequence Analysis 33
4 3 Results 38
95.5 KB
  3.1 Electrophoresis 38
  3.2 Sephadex Chromatography 38
  3.3 Electrophoresis Of Sephadex Peaks 43
  3.4 N-Terminal Analysis Of The Sephadex Peaks 43
  3.5 Ion-Exchange Chromatography 43
  3.6 Electrophoresis Of Cm-Cellulose 47
  3.7 N-Terminal Assay Of Peaks From Cm-Cellulose 47
  3.8 Purity Of Peaks On Hplc 47
  3.9 Amino Acid Composition 54
  3.10 Sequence Analysis Of S1 Cmb And S1cmc 54
5 4 Discussion 55
64.76 KB
  4.1 References 58
6 5 Part-II: Protein Patterns Of Human Cataractous Lens And Plasma 62
87.79 KB
  5.1 Introduction Lens/ Distribution And Etiology Of Cataract 62
  5.2 Senile Cataract 63
  5.3 Development Of Normal Lens 63
  5.4 Physical Characteristics Of The Normal Lens 64
  5.5 Function Of Lens 64
  5.6 Protein Constituents Of Lens 65
  5.7 Amino Acid Composition Of Lens 67
  5.8 Changes In Cataract Lens 67
7 6 Plasma / General Description 71
96.98 KB
  6.1 Amino Acids Of The Plasma 72
  6.2 Plasma Proteins 73
  6.3 Electrophoretic Studies 81
  6.4 Plasma Protein Changes In Diseased Subjects 83
  6.5 Object Of The Present Study 84
8 7 Materials And Methods 86
58.96 KB
  7.1 Sample Collection 86
  7.2 Preparation Of The Samples For Analysis 86
  7.3 Experimental Analysis 87
9 8 Results 93
88.26 KB
  8.1 Electrophoresis 93
  8.2 Sds-Page 98
  8.3 Iso-Electric Focusing 98
  8.4 Amino Acid Analysis 106
10 9 Discussion 107
81.86 KB
  9.1 References 110