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PART-1: CHARACTERIZATION OF HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICKII PART-II: PROTEIN PATTERNS OF HUMAN CATARACTOUS LENS AND PLASMA

Lalani, Rukhsana (1984) PART-1: CHARACTERIZATION OF HEMOGLOBIN FROM THE REPTILE UROMASTIX HARDWICKII PART-II: PROTEIN PATTERNS OF HUMAN CATARACTOUS LENS AND PLASMA. PhD thesis, University of Karachi, Karachi.

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Abstract

The present study on hemoglobin of Uromastix hardwickii has been undertaken to separate and characterize hemoglobin components. The separation of hemoglobin components was achieved by subjecting it to Sephadex G-75 column chromatography, ion-exchange chromatography using different resins and variety of buffers. The separation on molecular weight basis gave four peaks but when their homogeneity was checked, it was found that all the four peaks were not pure components. The separation on charge basis resolved into number of peaks. Checking the purity of these peaks by triton electrophoresis and N-terminal analysis showed only two of them to be pure. The purity of the same were checked on HPLC showing them to be homogeneous. However, the amino acid sequence using DABITC method could be achieved only to a limited number

Item Type:Thesis (PhD)
Uncontrolled Keywords:hemoglobin, reptile uromastix hardwickii, protein patterns, human cataractous lens, plasma, hemoglobin concentration, senile cataract
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:1285
Deposited By:Mr. Muhammad Asif
Deposited On:27 Jan 2007
Last Modified:04 Oct 2007 21:05

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