I= INTERACTION STUDIES OF CLARITHROMYCIN, ERYTHROMYCIN AND ROXITHROMYCIN WITH ESSENTIAL AND TRACE ELEMENTS
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Title of Thesis
INTERACTION STUDIES OF CLARITHROMYCIN, ERYTHROMYCIN AND ROXITHROMYCIN WITH ESSENTIAL AND TRACE ELEMENTS

Author(s)
Rizwana Sabri
Institute/University/Department Details
University of Karachi
Session
2004
Subject
Number of Pages
419
Keywords (Extracted from title, table of contents and abstract of thesis)
clarithromycin, erythromycin, roxithromycin, gram positive bacteria, gram negative bacteria, penicillinase, staphylococci, staphylococci, macrolide antibiotics

Abstract
The awareness of drug interaction is of obvious interest to health care professionals. The present work comprises of interaction studies of most commonly used macrolides i.e., erythromycin, clarithromycin and roxithromycin with various essential and trace elements, and H-2receptor antagonist such as cimetidine, ranitidine, famotidine and nizatidine.

These macrolide antibiotics are active agairst a wide range of Gram positive and Gram negative bacteria and other infectious agents, including penicillinase producing Staphylococci, and Staphylococci, and species [22, 25]. Erythromycin is a broad spectrum antibiotic with activity against Chlamydia trachomatis, Mycoplasma, pneumoniae, Ureaplasma, Urealyticllm, Spirochetes, Treponema pallidllm and Borrelia species [19]. Clarithromycin is a derivative of erythromycin, which also shows in vitro activity against many Gram-positive and Gram-negative aerobic and anaerobic organisms. The minimum inhibitory concentration (MIC) is generally two to four fold lower than that of erythromycin against Gram-positive bacteria, such as methicillin-sensitive Staphylococcus aureus and most Streptococcus species. Clarithromycin is bactericidal against Staphylococcus pyogenes, Staphylococcus pneumoniae and effective against Mycobacterium avium complex in human macrophages. Clarithromycin has greater in vitro activity than erythromycin against Legionella pnellmophi/a, Moraxel/a (Branhamella) catarrhalis, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma pneumoniae and Chlamydia trachomatis [378-379]. Roxithromycin is also a derivative of erythromycin, which shows similar range of activity to the later but is much more active in vivo [556]. Since the presence of complexing ligand may affect the bioavailability of metal in the blood or tissues therefore, the probable interaction of these macrolide were studied with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium metal salts. in vitro. All the reaction conditions were simulated to body environments and beyond to perform extended temperature studies.

The effect of pH on drug-metal complexation was ~so studied. These studies were carried out in buffers ranging from pH 1-3, in variable ratios of drug: metal, clarithromycin and roxithromycin being insoluble in pH 4-9. The interactions of all macrolide;; with metals resulted in decrease in absorbtion of drug in presence of all metals and pH studied. The stability of these complexes were determined in order to evaluate their possible in vivo implications. In order to investigate the number of metal ions involved in complexation with each macrolide, method of continuous variation was adopted.

Synthesis of metal complexes of erythromycin, clarithromycin and roxithromycin. were carried out with the salts of above stated elements. The structures of these complexes were elucidated by exploiting various analytical techniques such as 'H-NMR, infrared, ultraviolet studies, conductance measurement, the metals incorporated in the complexes analysed by atomic absorbtion and finally x-ray diffraction analysis of magnesium an:! manganese complexes of clarithromycin has proved that the drug molecule serves as a monodentate ligand coordinating through tertiary nitrogen at desosamine.

Drug interaction studies of these macrolides has also been carried out with H,-receptor antagonists. Macrolide and H2-receptor antagonists are commonly coadministered in patients complaining of GI irritation, acidity or ulcerative colitis. The behavior of erythromycin, clarithromycin and roxithromycin in presence of H2-receptor antagonists such as cimetidine, ranitidine, famotidine and nizatidine were studied in simulated gastric juice (pH I), buffers of pH 4,7.4 and 9 at 37, 48 and 60°C by using standard dissolution test apparatus. The percentage of each drug available at various time intervals was quantitated by measuring the absorbance at the maxima of each drug and using simultaneous equation. It has been established that macrolides also interact \\ith these H,-receptor antagonists.

To evaluate the changes in microbiological activity of these drugs after complexation with various essential and trace elements, antibacterial studies of these complexes were carried out against both Gram positive and Gram negative organisms such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella typhi, Proteus vulgaris, Shigella dysentery, Kelebcilla pneumoni and Staphylococcus epidermidis.

The changes in MIC of the complexes were compared with the parent drugs by measuring the zone of inhibition of these complexes. For MIC observation, agar dilution method was employed and zone series were determined by disk diffusion method. Our investigations divulge that erythromycin and clarithromycin metal complexes result in synergistic effects whereas roxithromycin metal complexes result in antagonistic effect.

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S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
267.46 KB
2 1 Introduction 1
978.46 KB
  1.1 Macrolides 6
  1.2 Erythromycin 8
  1.3 Clarithromycin 18
  1.4 Roxithromycin 34
  1.5 Trace And Essential Elements 43
  1.6 Drugs Acting As H 2 – Receptors Antagonists 54
  1.7 Objectives Of Present Work 62
3 2 Experimental 64
288.52 KB
  2.1 Materials 64
  2.2 Methods 65
4 3 Results & Discussion 83
3704.96 KB
  3.1 Macrolide Antibiotics 83
  3.2 H 2 -Receptor Antagonists 84
  3.3 Methods O Analysis 84
  3.4 In Vitro Availability Studies Of Macrolides And H 2 -Receptor Antagonists 85
  3.5 Simultaneous Determination Of Macrolides And H 2 -Receptor Antagonists 87
  3.6 Erythromycin, Clarithromycin And Roxithromycin Interactions With H 2 -Receptor Antagonists 88
  3.7 Erythromycin, Clarithromycin And Roxithromycin Metal Interactions The Effect Of Ph On Drug Metal Complex 101
  3.8 Job’s Method (Method Of Continuous Variation ) 101
  3.9 Synthesis Of Complexes Of Erythromycin, Clarithromycin And Roxithromycin With Essential And Trace Elements 320
  3.10 References 384