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Title of Thesis
HISTOCHEMCIAL CHANGES IN THE NEUROTRANSMITTERS OF WHITE RAT AND MONKEY HIND LIMB USING MESULAM TECHNIQUE |
Author(s)
Dr. Muhammad Zahoor Janjua |
Institute/University/Department Details
University of Karachi |
Session
1997 |
Subject
Anatomy |
Number of Pages
221 |
Keywords (Extracted from title, table of contents and abstract of thesis)
neurotransmitters, mesulam technique, neurons, lumbosacral enlargement, common peroneal (cpn), tibial (tn) nerves, femoral (fn) nerve |
Abstract The present study showed that using Elliott’s method, six major groups of neurons can be identified in the lumbosacral enlargement of both rats and monkeys. Horseradish peroxides method of neuronal labeling has revealed a striking similarity in the topographic localization and segmental distribution of the motoneurons forming the sciatic (ScN) and its components common peroneal (CPN) and tibial (TN) nerves, and the femoral (FN) nerve in neonatal, immature and adult rats and monkeys. The FN neurons were located more rostrally to the ScN neurons, the CPN neurons, the CPN neurons occurred more peripherally and slightly more rostrally to the TN neurons. The size spectrum, somatotopic organization and segmental distribution of the sensory, motor and postganglionic sympathetic neurons whose axons run in the CPN and TN correlated with the diameter spectrum of their myelinated nerve fibres showed a similar pattern of organization except for minor differences in the rates and monkeys.
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Chapter |
Title of the Chapters |
Page |
Size (KB) |
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| 1 |
0 |
Contents |
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 122.1 KB |
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| 2 |
1 |
Preface |
1 |
 782.74 KB |
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1.1 |
Introduction |
4 |
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1.2 |
Scope of Present Study |
5 |
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1.3 |
Review of Literature |
7 |
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| 3 |
2 |
Materials And Methods |
55 |
 352.63 KB |
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2.1 |
Animals and Their Care |
55 |
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2.2 |
Method of Anaesthesia |
58 |
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2.3 |
Operative Procedure & Application of Neuronal Tracer |
60 |
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2.4 |
Transaction of Spinal Cord and HRP Application |
62 |
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2.5 |
Study of Columnar Organization in Group I Animals |
64 |
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2.6 |
Elliott’s Method |
66 |
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2.7 |
HRP method in Group II A & B Animals |
67 |
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2.8 |
Double Labeling Method in Group IIC Animals |
71 |
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2.9 |
Localization of Neurons forming CPN & TN in rat (Group IIIA) |
72 |
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2.10 |
Localization of Neurons forming CPN & TN in Monkey (Group IIIB) |
74 |
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2.11 |
Epoxy resin sectioning |
75 |
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2.12 |
Data analysis |
76 |
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| 4 |
3 |
Results and Observations |
79 |
 388.02 KB |
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3.1 |
Columnar Organization in Group I Animals |
79 |
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3.2 |
Labelled motoneurons in rats in Group IIA Animals |
81 |
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3.3 |
Labelled Motoneurons in Monkeys in Group IIA Animals |
88 |
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3.4 |
Labelled Cervical Cord Neurons in Group IIB Animals |
92 |
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3.5 |
Double Labeling Techniques in Group IIC Animals |
93 |
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3.6 |
Labelled neurons forming CPN & TN in rat (Group IIIA) |
94 |
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3.7 |
Labelled neurons forming CPN & TN in monkey (Group IIIB) |
101 |
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| 5 |
4 |
Discussion |
108 |
 439.64 KB |
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| 6 |
5 |
Summary and Conclusions |
138 |
 87.79 KB |
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| 7 |
6 |
References |
144 |
 465.73 KB |
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| 8 |
7 |
Figures |
179 |
 422.74 KB |
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| 9 |
8 |
Tables |
218 |
 83.42 KB |
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| 10 |
9 |
Appendices |
i-xiii |
 137.29 KB |
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