I= HISTOCHEMCIAL CHANGES IN THE NEUROTRANSMITTERS OF WHITE RAT AND MONKEY HIND LIMB USING MESULAM TECHNIQUE
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Title of Thesis
HISTOCHEMCIAL CHANGES IN THE NEUROTRANSMITTERS OF WHITE RAT AND MONKEY HIND LIMB USING MESULAM TECHNIQUE

Author(s)
Dr. Muhammad Zahoor Janjua
Institute/University/Department Details
University of Karachi
Session
1997
Subject
Anatomy
Number of Pages
221
Keywords (Extracted from title, table of contents and abstract of thesis)
neurotransmitters, mesulam technique, neurons, lumbosacral enlargement, common peroneal (cpn), tibial (tn) nerves, femoral (fn) nerve

Abstract
The present study showed that using Elliott’s method, six major groups of neurons can be identified in the lumbosacral enlargement of both rats and monkeys.

Horseradish peroxides method of neuronal labeling has revealed a striking similarity in the topographic localization and segmental distribution of the motoneurons forming the sciatic (ScN) and its components common peroneal (CPN) and tibial (TN) nerves, and the femoral (FN) nerve in neonatal, immature and adult rats and monkeys. The FN neurons were located more rostrally to the ScN neurons, the CPN neurons, the CPN neurons occurred more peripherally and slightly more rostrally to the TN neurons.

The size spectrum, somatotopic organization and segmental distribution of the sensory, motor and postganglionic sympathetic neurons whose axons run in the CPN and TN correlated with the diameter spectrum of their myelinated nerve fibres showed a similar pattern of organization except for minor differences in the rates and monkeys.

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2922.98 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
122.1 KB
2 1 Preface 1
782.74 KB
  1.1 Introduction 4
  1.2 Scope of Present Study 5
  1.3 Review of Literature 7
3 2 Materials And Methods 55
352.63 KB
  2.1 Animals and Their Care 55
  2.2 Method of Anaesthesia 58
  2.3 Operative Procedure & Application of Neuronal Tracer 60
  2.4 Transaction of Spinal Cord and HRP Application 62
  2.5 Study of Columnar Organization in Group I Animals 64
  2.6 Elliott’s Method 66
  2.7 HRP method in Group II A & B Animals 67
  2.8 Double Labeling Method in Group IIC Animals 71
  2.9 Localization of Neurons forming CPN & TN in rat (Group IIIA) 72
  2.10 Localization of Neurons forming CPN & TN in Monkey (Group IIIB) 74
  2.11 Epoxy resin sectioning 75
  2.12 Data analysis 76
4 3 Results and Observations 79
388.02 KB
  3.1 Columnar Organization in Group I Animals 79
  3.2 Labelled motoneurons in rats in Group IIA Animals 81
  3.3 Labelled Motoneurons in Monkeys in Group IIA Animals 88
  3.4 Labelled Cervical Cord Neurons in Group IIB Animals 92
  3.5 Double Labeling Techniques in Group IIC Animals 93
  3.6 Labelled neurons forming CPN & TN in rat (Group IIIA) 94
  3.7 Labelled neurons forming CPN & TN in monkey (Group IIIB) 101
5 4 Discussion 108
439.64 KB
6 5 Summary and Conclusions 138
87.79 KB
7 6 References 144
465.73 KB
8 7 Figures 179
422.74 KB
9 8 Tables 218
83.42 KB
10 9 Appendices i-xiii
137.29 KB