I= STUDIES ON THE STRUCTURAL MODIFICATIONS OF THE STEROIDS AND RELATED COMPOUNDS BY MICROORGANISMS
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Title of Thesis
STUDIES ON THE STRUCTURAL MODIFICATIONS OF THE STEROIDS AND RELATED COMPOUNDS BY MICROORGANISMS

Author(s)
Sadia Sultan
Institute/University/Department Details
University of Karachi/ Department of Chemistry
Session
2004
Subject
Chemistry
Number of Pages
230
Keywords (Extracted from title, table of contents and abstract of thesis)
steroids, microorganisms, bioactive steroids, biotransformation, microbial transformations, bioactivities, inhibitory activities, anti-inflammatory activity

Abstract
The work embodied in this dissertation comprises on three parts. Part-A describes the microbial transformations of five bioactive steroids, i.e., mesterolone (70), 17a-ethynyl-17B-hydroxy-4-androsten-3-one (81), 17a-ethyl-17B-hydroxy-4-androsten-3-one (82), cortisol (8) and (+)-androst-4-ene-3, 17-dione (4). Part-B describes the biotransformation of a seaquiterpene cedryl acetate (100). Part-C describes the bioactivities of substrates 70, 81, 82, 8, 4 and 100 and their microbial and chemical derivatives. It is further divided into two parts. Part C-1 explains the inhibitory activities of microbial and chemical derivatives of starting materials 81, 82, 8, 4 and 100 against the enzymes tyrosinase, prolyl endopoptidase and a-glucosidase while Part-C-II deals with the anti-inflammatory activity of mesterolone (70) and its derivatives.

Part_A: This part of the thesis is mainly concerned with the structural modification and characterization of steroids through microorganisms. The transformed products were either new or previously reported in the literature. They were characterized by various sophisticated spectroscopic techniques available at this institute

Mesterolone (70), on incubation with cephalosporium aphidicola, Rhizopus stolonifer and Fusarium lini, afforded six new and three known metabolites namely methyl-5a-androstan-3, 17-dione (71), 3B, 17B-dihydroxy-1a-methyl-5a-androstane (72) 1a-methyl-androst-1-ene-3, 17-dionw (73). 15a-hydroxy-1a-methydroxy-1a-methyl-5a-androst-3-one (77), 11a, 17B-dihydroxy-1a-methyl-5a-androstan-3-one (78) 15a, 17B-dihyroxy-1a-methyl-5a-androstane-3-one (79) and 15a, 17B-dihydroxy-1a-methyl-androst-1-en-3-one (80)

Download Full Thesis
2039.26 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
175.66 KB
2 1 Introduction 1
237.53 KB
3 2 Results And Discussion 29
1154.24 KB
4 3 Experimental 147
444.58 KB
  3.1 Microorganisms 148
  3.2 Preparation Of Media 148
  3.3 General Experimental Details 150
  3.4 Incubation Of The Substrates 152
5 4 Biological Activities 194
157.62 KB
  4.1 Enzyme Inhibitory Activities 195
6 5 Bibliography 214
105.9 KB
7 6 Glossary 226
34.01 KB
8 7 List Of Publications 229
14.3 KB