Pakistan Research Repository

ISOLATION AND BIOTRANSFORMATION OF SOME BIOACTIVE COMPOUNDS FROM MEDICAL PLANTS

Gondal, Humaira Yaseen (2004) ISOLATION AND BIOTRANSFORMATION OF SOME BIOACTIVE COMPOUNDS FROM MEDICAL PLANTS. PhD thesis, University of Karachi, Karachi.

[img]HTML
13Kb

Abstract

The work presented in this Ph.D dissertation is divided in three parts. Part-A deals with phytochemical studies on suregada multiflora, a medicinal plant of Bangladesh, Part-B deals with photochemical studies on Verbascum sinaiticum, while microbial transformations of some natural compounds are discussed in Part-C. Part-A, This part of thesis deals with photochemical investigation on the leaf extracts of Suregada multiflora. The crude extract of Suregada multiflora was found to possess selective and significant cytotoxicity against a 60-cell line tumor panels at the isolation of eleven different tetracyclic diterpene lactores 1-11, which are the members of a rare class of abiatene diterpene lactrones along with a flavonoid glycoside and a lignan Compounds 7-11 (Gelomulide F, D, E, A and G), isolated from suregada multiflora were evaluated in the primary anticancer assay of 3-cell lines at the National Cancer Institute (NCI) Bethesda, USA. Out of them compound 9 (Gelomulide E) showed promising activity against the NCI-H460 (Lung) cell line, with a growth inhibition of over 85% at concentration of 5X10-5M New diterpene lactones from suregada multiflora Six new compounds (1-6) have been isolated from the Suregada multiflora. Among them compounds 1 and 2 contain an epoxy linkage between C-8 and C-14, whereas compounds 3-5 were identified as 8-14-dihydroxy analogues of diterpene lactones. Compound 6 contains one additional epoxy ring between C-11 and C-12

Item Type:Thesis (PhD)
Uncontrolled Keywords:bioactive compounds, medical plants, suregada multiflora, verbascum sinaiticum, microbial transformations, anticancer assay
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:1080
Deposited By:Mr. Muhammad Asif
Deposited On:19 Feb 2007
Last Modified:04 Oct 2007 21:04

Repository Staff Only: item control page