I= PRIMARY METABOLITES OF CANDIDA TROPICALIS
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Title of Thesis
PRIMARY METABOLITES OF CANDIDA TROPICALIS

Author(s)
Atiya Abbasi
Institute/University/Department Details
University of Karachi/ H.E.J Research Institute of Chemistry
Session
1983
Subject
Chemistry
Number of Pages
91
Keywords (Extracted from title, table of contents and abstract of thesis)
candida tropicalis, b-lactoglobulin , valinease, enzymic activity, enzymes, proteins, polysaccharides, lipids, synthetic peptides

Abstract
This is the first study to the characterization of proteins and peptides in the cell free broth of Candida tropicalis. We are reporting the presence of a highly specific proteolytic enzyme cleaving at valine. This has been confirmed by digesting B-lactoglobulin and a number of synthetic peptides. The enzyme has a carbohydrate moiety and a molecular weight of 40,000+7,000 as determined on SDS-plyacrylamide gel electrophoresis. Its optimum activity occurs at 37C at a ph between 8-9. We have named it Valinease because of its selective cleavage

The peptides separated on Dowex 50 have been tested against a number of pathogenic and non-pathogenic bacteria. At least four of the peptides have been found to be active against most of the bacterial strains. The amino acid composition and N-terminal residues of these peptides have been determined

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41487.5 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
1914.81 KB
2 1 Introduction 1
11438.51 KB
  1.1 General Background 1
  1.2 Enzymes 3
  1.3 Proteins 7
  1.4 Polysaccharides 11
  1.5 Lipids 14
  1.6 Miscellanous 15
  1.7 Biological Aspect 19
  1.8 Pathogenesis by Ctropicalis 21
  1.9 Experimentally induced Candidiasis in Animals 24
  1.10 Objectives of present study 25
3 2 Experimental 26
11321.89 KB
  2.1 Composition of Culture media 26
  2.2 Preparation of culture media 28
  2.3 Growth of the culture 31
  2.4 Isolation of components from cell free broth 31
  2.5 Gel filtration chromatography 32
  2.6 Electgrophoresis 33
  2.7 Affinity Chromatography 36
  2.8 Enzyme assay 37
  2.9 Oxidation with performic acid 37
  2.10 Ion-Exchange chromatography 38
  2.11 Amino acid analysis 39
  2.12 Paper chromatography 44
  2.13 Anti-Microbial assay 46
  2.14 N-Terminal assay 48
  2.15 Carbohydrate analysis 49
4 3 Results 53
8810.56 KB
  3.1 Isolation of proteins and peptides 53
  3.2 Electrophoresis of Proteins 53
  3.3 Separation of proteins/peptides on sephadex 57
  3.4 Purificatrion of enzyme 57
  3.5 Electrophoresis of CTEpS1 59
  3.6 Amino acid composition of CTEpS1 59
  3.7 N-Terminal of CTEpS1 61
  3.8 Carbohydrate contents of CTEpS1 61
  3.9 Enzyme activity of CTEpS1 62
  3.10 Effect of ph on enzymic activity 64
  3.11 Effect of temperature on enzymic activity 64
  3.12 Splitting of B-lactoglogulin 66
  3.13 Splitting of synthetic peptides 69
  3.14 Separation of CT-Ep-F on dowex 50X2 71
  3.15 Amino acid analysis of peptides 71
  3.16 N-Terminal assay of peptides 73
  3.17 Antimicrobial activity of peptides 73
5 4 Discussion 76
8161.93 KB
  4.1 Chemical Aspect 76
  4.2 Biological aspect 81
  4.3 References 94