Pakistan Research Repository


Abbasi, Atiya (1983) PRIMARY METABOLITES OF CANDIDA TROPICALIS. PhD thesis, University of Karachi, Karachi.



This is the first study to the characterization of proteins and peptides in the cell free broth of Candida tropicalis. We are reporting the presence of a highly specific proteolytic enzyme cleaving at valine. This has been confirmed by digesting B-lactoglobulin and a number of synthetic peptides. The enzyme has a carbohydrate moiety and a molecular weight of 40,000+7,000 as determined on SDS-plyacrylamide gel electrophoresis. Its optimum activity occurs at 37C at a ph between 8-9. We have named it Valinease because of its selective cleavage The peptides separated on Dowex 50 have been tested against a number of pathogenic and non-pathogenic bacteria. At least four of the peptides have been found to be active against most of the bacterial strains. The amino acid composition and N-terminal residues of these peptides have been determined

Item Type:Thesis (PhD)
Uncontrolled Keywords:candida tropicalis, b-lactoglobulin , valinease, enzymic activity, enzymes, proteins, polysaccharides, lipids, synthetic peptides
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:1056
Deposited By:Mr. Muhammad Asif
Deposited On:19 Dec 2006
Last Modified:04 Oct 2007 21:04

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