Pakistan Research Repository

PRIMARY METABOLITES OF CANDIDA TROPICALIS

Abbasi, Atiya (1983) PRIMARY METABOLITES OF CANDIDA TROPICALIS. PhD thesis, University of Karachi, Karachi.

[img]HTML
16Kb

Abstract

This is the first study to the characterization of proteins and peptides in the cell free broth of Candida tropicalis. We are reporting the presence of a highly specific proteolytic enzyme cleaving at valine. This has been confirmed by digesting B-lactoglobulin and a number of synthetic peptides. The enzyme has a carbohydrate moiety and a molecular weight of 40,000+7,000 as determined on SDS-plyacrylamide gel electrophoresis. Its optimum activity occurs at 37C at a ph between 8-9. We have named it Valinease because of its selective cleavage The peptides separated on Dowex 50 have been tested against a number of pathogenic and non-pathogenic bacteria. At least four of the peptides have been found to be active against most of the bacterial strains. The amino acid composition and N-terminal residues of these peptides have been determined

Item Type:Thesis (PhD)
Uncontrolled Keywords:candida tropicalis, b-lactoglobulin , valinease, enzymic activity, enzymes, proteins, polysaccharides, lipids, synthetic peptides
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:1056
Deposited By:Mr. Muhammad Asif
Deposited On:19 Dec 2006
Last Modified:04 Oct 2007 21:04

Repository Staff Only: item control page