Pakistan Research Repository

CHARACTERIZATION OF VENOM PROTEINS FROM PSEUDOCERASTES PERSICUS PERSICUS

Mirzajiani, Farzaneh (1999) CHARACTERIZATION OF VENOM PROTEINS FROM PSEUDOCERASTES PERSICUS PERSICUS. PhD thesis, University of Karachi, Karachi.

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Abstract

Psuedocerastes persicus persicus (false horn viper) snake venom and its gel filtration fractions were tested for a number of biological tests. Results from biological studies indicated that venom from Psuedocerastes persicus persicus contains number of toxic, haemorrhagic, proteolytic, anticoagulant, cytotoxic and enzymatic proteins. Venom did not show either haemolytic or apoptotic activity and also there was no evidence of presence of growth factors in crude venom. Among all enzymes activities tests in curde venom i.e LDH, ALT, SOD, Catalase, ALP, CPK, AST, a-Amylase, PDE, Endonuclease and L-amino acid oxidase actgivity, very high activity of LAO and moderate activity of LDH, ALP, and a-Amylase was detected in crude venom The major lethal factor showing strong L-amino acid oxidase activity isolated from the venom of Psuedocerastes persicus persicus by a combination of gel filtration on Sephadex G-50, Sephadex G-100 and heparin affinity chromatography, is a homodimer protein with approximate mol. Wt. 13.000 Da as determined by SDS-? AGE. It is the main portion of total venom protein and exhibits potent lethal, heamorrhagic and cytotoxic activity The enzyme exhibited unusual thermal stability and unlike most other venom LAO’s it was stable in alkaline solution and was inactivated by freezing. Kinetics studies proved that this enzyme is new isoform of L-amino acid oxidase Cytotoxicity effect of venom and its fractions on many human and mouse cancerous cell lines including SK-CO-1, HT144, HI299, HT157, EBV-tranformed B-lymphocytes, SP2, and normal B-lymphocytes, shows that the extent of cytotoxicity effect of venom compounds is variable depending on cell lines. Cytotoxicity of crude venom and fraction (F1) containing L-amino acid oxidase was mostly inhibited by catalase, indicating that H2O2 produced by LAO is the main cause pf sutptpxocoty by venom. These data suggests the treatment with anti-oxidants for protecting tissues affected by snake bite No apoptotic effect was detected in a number of experiments carried out on human cancerous cells (SK-CO-1 and HT144) and B-lymphocytes exposed to the different concentration of crude venom and F1 (L-amino acid oxidase), indicating that LAO is capable of inducing apoptosis in some particular cell lines but not in all cell lines

Item Type:Thesis (PhD)
Uncontrolled Keywords:venom, proteins, false horn viper, snake, psuedocerastes persicus persicus, enzymes, haemorrhagic proteins, proteolytic proteins, anticoagulant proteins, cytotoxic proteins, enzymatic proteins, homodimer protein
Subjects:Physical Sciences (f) > Chemistry(f2)
ID Code:1052
Deposited By:Mr. Muhammad Asif
Deposited On:19 Dec 2006
Last Modified:04 Oct 2007 21:04

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