I= SEED-BORNE MYCOFLORA OF IMPORTANT CROP PLANTS WITH SPECIAL REFERENCE TO TOXIGENIC SPECIES OF FUSARIUM
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Title of Thesis
SEED-BORNE MYCOFLORA OF IMPORTANT CROP PLANTS WITH SPECIAL REFERENCE TO TOXIGENIC SPECIES OF FUSARIUM

Author(s)
Fakhrum-Nisa
Institute/University/Department Details
University of Karachi
Session
1998
Subject
Botany
Number of Pages
102
Keywords (Extracted from title, table of contents and abstract of thesis)
seed-borne mycoflora, fusarium, wheat, corn, millet, sorghum, barley, fusarium proliferatum, f.subglutinans, stemphylium sp., aspergillus sulphureus, a.candidus, drechslera halodes, rhizoctonia solani, f.subglutinans, d.halodes, f.equiseti, f.clamydosporum, f.longipes, f.proliferatum, f.subglutinans, f.pallidoroseum, f.solani, chalara sp., choanephora sp., d.tetramera, trichothecium sp., cladosporium herbarum, trichoderma sp., cephalosporium sp., stemphylium sp., f.subglutinans, piptocephalis sp., and syncephalastrum recemosum, aspergillus niger, cephalosporium sp., cladosporium herbarum, drechslera dematioidea, stemphylium sp., ulocladium sp.

Abstract
Seed samples of wheat, corn, millet, sorghum and barley obtained from various parts of Pakistan were studied for seed-borne mycoflora by simple blotter method and deep freezing method. Of the seed-home fungi, 12 genera and 21 species from 19 samples of wheat, 10 genera and 20 species from 35 samples of corn, 15 genera and 31 species from 25 samples of mille., 14 genera and 23 species on 27 samples of sorghum and 11 genera and 17 species from 14 samples of barley were isolated and identified. Fusarium proliferatum, F.subglutinans, Stemphylium sp., Aspergillus sulphureus, A.candidus and Drechslera halodes on corn; Rhizoctonia solani and F.subglutinans on wheat; D.halodes, F.equiseti, F.clamydosporum, F.longipes, F.proliferatum, F.subglutinans, F.pallidoroseum, F.solani, Chalara sp., Choanephora sp., D.tetramera, Trichothecium sp., Cladosporium herbarum, Trichoderma sp., Cephalosporium sp, and Stemphylium sp., on millet; F.subglutinans, Piptocephalis sp., and Syncephalastrum recemosum on sorghum and Aspergillus niger, Cephalosporium sp., Cladosporium herbarum, Drechslera dematioidea, Stemphylium sp., and Ulocladium sp., on barley are new records from Pakistan. Phytotoxic effect of Fusarium spp., on seedling growth of wheat, corn, millet and sorghum, and on leaves and roots of wheat seedlings was observed. Effect of Fusarium culture filterates decreased growth of seedlings. The effect of temperature, moisture content and storage period on seed-borne mycoflora was examined where 19% moisture content of barley and 13% of wheat, corn and sorghum at 20oC was found to be most favourable for the growth of storage fungi. Interaction of Fusarium spp., with other fungi in vitro was also studied. In dual culture plate assays, Trichoderma hamatum showed inhibition in growth of Fusarium oxysporum, Fpallidoroseum F.sporotrichioides, F.moniliforme, Fsubglutinans, F.proliferatum, F.equiseti, F.longipes, F.scirpi and F.solani producing zone of inhibition. Trichoderma hamatum showed antagonistic activity against Fusarium spp., in vivo.

Download Full Thesis
2275.14 KB
S. No. Chapter Title of the Chapters Page Size (KB)
1 0 Contents
72.33 KB
2 1 Summary I
27.72 KB
3 2 Introduction 1
45.92 KB
4 3 Literature Review 6
130.92 KB
5 4 Materials And Methods 21
115.97 KB
  4.1 Collection Of Seed Samples 21
  4.2 Isolation Of Seed Borne Mycoflora 21
  4.3 Media For Maintaining 22
  4.4 Phytotoxic Effect Of Fusarium Spp 24
  4.5 Interaction Of Fusarium Spp ., With Other Fungi In Vitro 26
  4.6 Interaction Of Fusarium Spp ., With Trichoderma Hamatum 29
  4.7 Storage Of Grains Under Laboratory Conditions 30
  4.8 Media For Production Of Mycotosins And Other Secondary Metabolites 31
  4.9 Preparation Of Tlc Plates 32
  4.10 Detection Of Mycotoxins And Pigments 33
  4.11 Thin Layer Chromatography 33
  4.12 Preparative Thin Layer Chromatography 34
  4.13 Growth Response Of Fusarium Spp ., On Tan Agar 35
6 5 Results 37
1339.34 KB
  5.1 Seed-Borne Mycoflora 37
  5.2 Phytoxoic Effect Of Fusarium Species 58
  5.3 Phytoxoic Effect On Seedling Growth 58
  5.4 Phytoxoic Effect On Seedlings Through Leaves 68
  5.5 Interaction Of Fusarium Spp ., With Other Fungi In Vitro 72
  5.6 Interaction Of Fusarium Spp ., With Trichoderma Hamatum In Vivo 86
  5.7 Effect Of Moisture, Temperature And Storage Period On Seed-Borne Mycoflora 88
  5.8 Mycotoxins And Other Secondary Metabolites Of Fusarium Spp 97
  5.9 Growth Response Of Fusarium Spp ., On Tan Agar 142
7 6 Discussion 151
96.96 KB
8 7 References 163
207.36 KB
9 8 Acknowledgements 194
498.86 KB
  8.1 Appendices 197
  8.2 Publications