Fakhrun, -Nisa (1998) SEED-BORNE MYCOFLORA OF IMPORTANT CROP PLANTS WITH SPECIAL REFERENCE TO TOXIGENIC SPECIES OF FUSARIUM. PhD thesis, University of Karachi, Karachi.
Seed samples of wheat, corn, millet, sorghum and barley obtained from various parts of Pakistan were studied for seed-borne mycoflora by simple blotter method and deep freezing method. Of the seed-home fungi, 12 genera and 21 species from 19 samples of wheat, 10 genera and 20 species from 35 samples of corn, 15 genera and 31 species from 25 samples of mille., 14 genera and 23 species on 27 samples of sorghum and 11 genera and 17 species from 14 samples of barley were isolated and identified. Fusarium proliferatum, F.subglutinans, Stemphylium sp., Aspergillus sulphureus, A.candidus and Drechslera halodes on corn; Rhizoctonia solani and F.subglutinans on wheat; D.halodes, F.equiseti, F.clamydosporum, F.longipes, F.proliferatum, F.subglutinans, F.pallidoroseum, F.solani, Chalara sp., Choanephora sp., D.tetramera, Trichothecium sp., Cladosporium herbarum, Trichoderma sp., Cephalosporium sp, and Stemphylium sp., on millet; F.subglutinans, Piptocephalis sp., and Syncephalastrum recemosum on sorghum and Aspergillus niger, Cephalosporium sp., Cladosporium herbarum, Drechslera dematioidea, Stemphylium sp., and Ulocladium sp., on barley are new records from Pakistan. Phytotoxic effect of Fusarium spp., on seedling growth of wheat, corn, millet and sorghum, and on leaves and roots of wheat seedlings was observed. Effect of Fusarium culture filterates decreased growth of seedlings. The effect of temperature, moisture content and storage period on seed-borne mycoflora was examined where 19% moisture content of barley and 13% of wheat, corn and sorghum at 20oC was found to be most favourable for the growth of storage fungi. Interaction of Fusarium spp., with other fungi in vitro was also studied. In dual culture plate assays, Trichoderma hamatum showed inhibition in growth of Fusarium oxysporum, Fpallidoroseum F.sporotrichioides, F.moniliforme, Fsubglutinans, F.proliferatum, F.equiseti, F.longipes, F.scirpi and F.solani producing zone of inhibition. Trichoderma hamatum showed antagonistic activity against Fusarium spp., in vivo.
|Item Type:||Thesis (PhD)|
|Uncontrolled Keywords:||seed-borne mycoflora, fusarium, wheat, corn, millet, sorghum, barley, fusarium proliferatum, f.subglutinans, stemphylium sp., aspergillus sulphureus, a.candidus, drechslera halodes, rhizoctonia solani, f.subglutinans, d.halodes, f.equiseti, f.clamydosporum, f.longipes, f.proliferatum, f.subglutinans, f.pallidoroseum, f.solani, chalara sp., choanephora sp., d.tetramera, trichothecium sp., cladosporium herbarum, trichoderma sp., cephalosporium sp., stemphylium sp., f.subglutinans, piptocephalis sp., and syncephalastrum recemosum, aspergillus niger, cephalosporium sp., cladosporium herbarum, drechslera dematioidea, stemphylium sp., ulocladium sp.|
|Subjects:||Biological & Medical Sciences (c) > Biological Sciences(c1) > Paleo-botany(c1.9)|
|Deposited By:||Mr. Muhammad Asif|
|Deposited On:||18 Dec 2006|
|Last Modified:||04 Oct 2007 21:04|
Repository Staff Only: item control page